Clarity is an important measure of seed quality. Clarity analysis of seeds can determine the weight percentages of different components of the test sample and the characteristics of the sample mixture, and infer the composition of the seed batch accordingly. Clarity analysis includes two aspects: First, determine the percentage of each component of the test sample. According to GB/T 3543.3-1995, the sample is divided into three components: net seeds, other plant seeds, and impurities. The results for each component are expressed in percent by weight. The second is to analyze the characteristics of the sample mixture. Clarity analysis not only calculates the weight percentage of each component, but also identifies the species to which other plant seeds and impurities in the sample belong. The number of seeds is often used in the analysis of seed cleanliness, often using a pelletizer or an automatic pelletizer.
1 Preparation before analysis
Before analysis, whether the sample number of the sample to be submitted for inspection is consistent with the record, whether the species is the species described in the record, whether the weight of the sample to be tested meets the requirements, and the inspection of heavy-duty contaminants is required.
1.1 Weighing for Inspection Samples
According to the requirements of GB/T 3543.2-1995, determine the minimum weight of the sample to be tested. The weight of the submitted sample is generally more than 10 times the amount of the clarity analysis.
1.2 Inspection of heavy-duty hybrids
Large-grained seeds, small stones, clods, and other heavy contaminants, which are significantly different in size and weight from the tested samples, are small in quantity and heavy in weight and seriously affect the analysis results. Before the Clarity Analysis, the heavy mixture must be picked from the test sample and weighed (m) before it is separated into other plant seeds and impurities and weighed as m1 and m2 (m1 + m2! = m respectively). ).
2 Dividing of test samples
2.1 Test sample weight requirements
Clarity test samples shall be taken from the test sample in accordance with the requirements of GB/T 3543.2-1995. The weight shall be estimated to contain at least 2500 seed units or not less than GB/T 3543.2-1995.
2.2 Take samples
Follow the sampling rule, and use the sampler to divide or quadruplicate method to decrement repeatedly until the test sample of the specified weight is dispensed. One or two half samples (half of the sample weight) of the specified weight are used for the clarity analysis.
2.3 Sample weighing
Weigh the sample with a scale that meets the requirements of both weighing and inductivity. The unit is expressed in g and accurate to the decimal places specified in the table below. Weighing and decimal places
3 sample separation, identification and weighing of each component
After the samples were weighed, the samples were divided into net seeds, other plant seeds, and impurities according to the standard of the net seeds. Separation can be done with the aid of magnifying glass, microscope, set of sieve, hair dryer, cleaning machine, electric screening machine, vibration separator, electro-optic inspection device and other appliances. A commonly used method is to select a suitable two-layer sieve with a sieve mesh. The pore size of the small pore sieve is required to be smaller than the analyzed seeds, and the pore size of the large pore sieve is larger than the analyzed seeds. When used, the small-hole screen is placed under the large-mesh screen, and then the screen box is placed under the small-hole screen, poured into the sample or half-sample, capped, placed on an electric screen or manually screened for 2 minutes. After sieving, the layers in the sieve and the bottom box are poured on the cleanliness analysis platform for analysis and identification. The net seeds, other plant seeds and impurities are separated, and the various components are weighed separately. The unit is denoted by g. .
4 Determination of the number of other plant seeds
Determination of the number of other plant seeds According to the different requirements of the sender, three different methods can be adopted: complete inspection, limited inspection and simplified inspection.
5 result calculation and data processing
5.1 Check the weight loss of the analysis process
Regardless of whether it is a sample or two and a half separate samples, the sum of the weights of the various components after analysis should be compared with the original weight, and whether or not the substance is increased during the analysis should be checked. If the gap between the increments exceeds 5% of the original weight, you must redo the results and fill in the redo results.
5.2 Calculate the weight percentage of each component
When using the whole sample analysis, the weight percentage of net seeds, other plant seeds, and impurities should be calculated to a decimal number; When using half sample analysis, each component of each half sample should be calculated separately, and the percentage is kept at least to two. Decimal, and calculate the average percentage of each component. The calculation of the percentage must be based on the sum of the weights of the various components after the analysis, not on the original weight of the test sample.
5.3 Checking Errors Between Repetitive Analyses
If two half-samples are analysed, the deviation of any component shall not exceed the permissible error between repeated analyses as shown in the permissible error table of the Clarity Analysis of GB/T 3543.3-1995. If the actual difference of all components is within the allowable range, Then calculate the average value of each component; if the actual error exceeds the allowable range, re-analyze the two and a half samples until it is within the allowable range.
5.4 Calculation Results and Data Correction
There are two ways to calculate the average value of the seed test results. One is the arithmetic mean; the other is the weighted average. The average of the cleanliness analysis is a weighted average, and the final result is the sum of the sum of the individual repeat components of the net seeds and the total weight after the analysis, and the other components are the same. The final result of each component is retained as a decimal. The weight percentage of each component is added, and its sum should be 100.0 %. Trace components less than 0.05 % should be excluded from the calculation. If the sum is not 100.0 % but 99.9 % or 100.1 %, it is increased or decreased by 0.1 % from the maximum value (generally net seed). If the rounding value is greater than 0.1%, check the calculation for errors.
6 Fill in the report of the results of the clarity analysis
The result of the clarity analysis is expressed as the weight percentage of the three components, accurate to one decimal place, and the sum of the percentages of the various components must be 100.0 %. Ingredients less than 0.05% are reported in small quantities; if one component has a result of 0, they are reported as “―0.0―â€. When it is found that the weight percentage of certain impurities or one of the other plant seeds reaches 1% or more, the species must be indicated on the result report form.
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